Resun Nanocoulter technology for solution background testing

 —Analyze the concentration of particles in solution A after wetting the experiment consumables

Experiment consumables such as centrifugal tubes and pipetting gun heads are widely and frequently used in scientific research. For some high-precision analysis experiments or high-end applications, the cleanliness of experiment consumables may be a very critical but easily overlooked factor. The Resun Nanocoulter technology can evaluate the cleanliness of the consumables by measuring the concentration of particles of the solution which the corresponding experiment consumables is soaked with.

The following is an analysis of particle concentrations in the infiltration fluid of centrifugal tubes, pipette tips and syringes purchased from different suppliers:

1.Centrifugal tubes

Centrifugal tubes:

1)Take 5 centrifugal tubes of different specifications from supplier T/J and supplier B respectively;

2)Add the diluent filtered by 100nm filter membrane into each centrifugal tube, shake the bed 3 times, shake well for 1 minute;

3)Wash the test components, and add the filtered diluent directly into the sample pool for testing to verify whether the components are clean;

4)Take clean test components, add the infiltrating centrifugal tube components into the sample pool quantitatively, and record the test;

5)Statistically analyze the data of infiltrating fluid of centrifugal tubes of different specifications from different suppliers.

Data analysis:

As shown in the bar chart below, we compare the cleanliness of 600μL, 1.5mL and 2mL centrifuges purchased from supplier T/J (the greater the concentration of infiltrating fluid particles, the dirtier the centrifuges). The centrifugal tubes provided by supplier -B are obviously superior to those of T/J.

Taking the 1500 microliter centrifugal tube provided by supplier B as an example, the particle size distribution figure of the diluent infiltrated onto the centrifugal tube is as follows: The particle size of the diluent infiltrated onto the 1500 microliter centrifugal tube is mainly distributed within 80-130nm.

2．Pipette tips (after analysis, here is a 200μL pipette tip)

Experiment scheme

1）Take ten 200 microliter pipette tips from supplier D, supplier T/J-1 and supplier T/J-2 respectively;

2）Install pipette tips with pipettes, drain the filtered diluent into a clean 5ml centrifuge tube (which has been verified to be clean), and collect the diluent of 10 pipette tips into the same centrifuge tube;

3）Add the collecting liquid from each supplier's pipette tips into the sample pool for testing;

4）Statistically analyze the data.

Data analysis

As shown in the figure below, there is little difference in the cleanliness of the pipette tips provided by the three suppliers, but the cleanliness of the pipette tips provided by supplier D is superior to that provided by supplier T/J.

According to the test data of the pipette tips infiltration diluent of supplier T/J-1, the distribution diagram of particle size in the liquid is as follows: After using the 200 microliter pipette tips of this supplier, the content particle size in the diluent is mainly distributed between 80-120nm, and more between 120-150nm.

3.Syringes (the analysis here is a 1mL syringe)

The piston surface of disposable sterile syringe is lubricated by polydimethylsiloxane (silicone oil). When the liquid containing surfactant is absorbed, the silicone oil will dissolve and form small particles in the solution. The content of silicone oil is determined by testing the content of small particles.

Among the four brands of syringes provided by supplier J of this test, the procedure is directly using the corresponding syringe to absorb diluent into the clean test component and analyze the data.

Data analysis
As shown in the figure below, the supplier's J-T syringe has *more particles after inhaling the diluent, while the supplier's J-H syringe has fewer particles. According to our analysis of the concentration and particle size of nanoparticles, the supplier's J-H syringe is the *best.

Taking the sample test data of syringe from supplier J-T as an example, the particle size distribution in diluent after syringe was analyzed, and the particle size distribution diagram is as follows.

As shown in the above figure, after the syringe is used to sample, the particle size in the diluent is mainly 70-150nm, and there are many particles in the range of 150nm-200nm.