Application of Nanocoulter I in Viral Vectors

I. Characteristics of Commonly-Used Viral Vectors

II. Application of Nanocoulter I after Viral Vector Purification

III. High-precision and Ultra-sensitive Detection Platform in the Fields of Bio-nano Drugs and Nanomaterials

1. Main Characteristics of Nanocoulter Ⅰ:

Ø Nanoparticles in fluids are detected individually  

Ø Disposable test card can avoid cross contamination

Ø The test process can be visualized by Multi-dimensional data

Ø The size distribution, concentration and potential data of nanoparticles are automatically analyzed without benchmarking

Ø Any type of nanoparticles (inorganic & organic, transparent & opaque, conductive & insulating)

Ø Particle size measurement range:  40-2000nm

Ø Concentration measurement range:  106-1012 particles/ml

Ø Detection speed:  The test is completed within 5 minutes

Ø Sample demand: Within 50μl

Ø Desktop size: 400(mm)*350(mm)*310(mm)

Ø Weight: 19kg

2. Coulter Principle (also known as: Resistive Pulse Sensing(RPS)): 

The particles suspended in the electrolyte displace the same volume of electrolyte as they pass through the pores with the electrolyte, leading to the instantaneous change of the resistance between the two electrodes inside and outside the pore tube in the designed constant current circuit to generate a potential pulse. The size and number of pulse signals are proportional to the size and number of particles. It belongs to the measurement of individual particles and three-dimensional measurement, which not only accurately measures the particle size distribution of materials, but also measures the precise number and concentration of particles. The measured particle size is closer to the real one without being affected by the physical and chemical properties of the sample.

 

3. Nanocoulter Ⅰ Lentivirus Test:

Test steps:

①The two samples including PC (before filtering) and P02 (after filtering) are used to characterize the difference between samples before and after filtering.

② The 2 samples were diluted with 004 diluent and tested to observe the distribution of large particles.

Fig. 1 (Exosome sample test data)

Fig. 2 (Experiment test report)

Test summary:

The concentration of large particles in exosome samples is around 4.77E+10, and about 60% of the particles will be lost after filtration by the filter membrane. Its concentration becomes around 1.65E+10, with a reduction in large particles and a corresponding reduction in mean particle size.

Single particle detection is performed to obtain the real particle size distribution of samples, which can accurately characterize the differences before and after samples being filtered through the filtration membrane.